RNA

Part:BBa_K1412089:Design

Designed by: Yahong Chen   Group: iGEM14_XMU-China   (2014-10-16)

Riboregulator which combines crRNA and RBS acting as a lock to the gene cuicuit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our project, we consider different kinds of keys can open our lock.Based on our project, we design the part based on the two principle(2):

1.The binding fore between taRNA and crRNA is stronger than that between crRNA and RBS or that between aptamer and taRNA.

2.RBS and AUG are separated by about 6 bases.


Source

References

1.Callura J M, Cantor C R, Collins J J. Genetic switchboard for synthetic biology applications[J]. Proceedings of the National Academy of Sciences, 2012, 109(15): 5850-5855.

2.Farren J Isaasc, Daniel J Dwyer, Ding C, Pervouchine DD, Cantor CR, Collins JJ, et al.Engineered riboregulators enable post-translational control of gene expression. Nat Biotechnol. 2004 Jul;22(7):841-7.